Bhau, B.S.

Wakhlu, A.K.

Knowledge Repository Open Network

 Author(S): B.S. Bhau & A.K. Wakhlu Title: Effect of genotype, explant type and growth regulators on organogenesis in Morus alba Keywords:  Auxin, Cytokinin, Genotype, Mature Explant, Morus, Mulberry, Organogenesis Year: 2001 Name of journal: Plant Cell, Tissue and Organ Culture Volume & Issue 66(1) Page No: 25-29 Institute: Plant Tissue Culture Laboratory, Department of Botany Jammu University, Jammu 180 006 Jammu & Kashmir,India.      DOI 10.1023/A:1010617212237 Abstract  Plantlets of the mulberry (Morus alba L. vars. Chinese White, and Kokuso-27) were produced from callus cultures. For callus induction, leaf, internodal segments, and petiole explants of Chinese White, Kokuso-27 and Ichinose varieties were grown on MS basal medium fortified with 2,4-D and 6-benzylaminopurine (BA). Callogenesis was dependent on the nature of explant used, the genotype and growth regulators supplemented in the medium. Leaves were the best explant type used for callus induction. Best callogenesis was obtained on MS medium containing a combination of 1 mg l−1 2,4-D and 0.5 mg l−1 BA (95-100%). Calluses formed shoots on MS medium supplemented with 1 mg l−1 BA. Supplementation with 0.1 mg l−1 2,3,5-triiodobenzoic acid (TIBA) in this medium enhanced shooting response. Presence of TIBA in the medium also improved the long-term organogenic potential of the callus. Regenerated shoots produced roots on Murashige & Skoog (MS) medium containing either 0.5 mg l−1 indole-3-butyric acid (IBA) or α-naphthaleneacetic acid (NAA). Seventy percent of the rooted plants were established in the field where they are performing well. 

Effect of genotype, explant type and growth regulators on organogenesis in Morus alba

http://dspaces.uok.edu.in/jspui//bitstream/1/1513/3/Bhau%20%26%20Wakhlu.pdf

Effect of genotype, explant type and growth regulators on organogenesis in Morus alba

Abstract

Similar works

Full text

Available Versions

Knowledge Repository Open Network