Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Candidate: Tereza Kubačková Supervisor: Doc. PharmDr. František Trejtnar, CSc Title of diploma thesis: Study of membrane transport of xenobiotics in vitro Some human endocrinal tumors overexpress peptide specific receptors. This discovery forms the basis of the conception of the usage receptor specific peptide-based radiopharmaceuticals for diagnostic imagining and more recently, for receptor-targeted radiotherapy. However, considering accumulation and retention in renal tissue, these substances might cause the radiotoxic damage of kidneys. So nephrotoxicity is a factor restricting the application of radiopharmaceuticals in therapy, because only a limited amount of radiopharmaceutical doses could be used. The aim of the study was to investigate the rate of accumulation and the renal uptake mechanisms of the new gastrin derivate - DOTA-minigastrin 11 (DOTA-MG11) 111indium- labeled in the cellular in vitro model. Properties of 111 In-DOTA-MG11 were confronted with these in substances from another group of receptor specific radiopeptides - somatostatin analogs such as 111 In-DOTA-naphtyl-octreotide (111 In-DOTA-NOC) and 111 In-DOTA- octreotate (111 In-DOTA-TATE). For quantitative comparison of..
