Rosíková Pavla Evaluation of activity of potentional antifungal substances through the use of microdilution broth method II Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Kralove Pharmacy The aim of this diploma thesis was testing of samples of substances produced at the Department of Pharmaceutical Chemistry and Drug Control and the Department of Inorganic and Organic Chemistry Faculty of Pharmacy of Charles University in Hradec Kralove. Substances were tested by using microdilution broth method. Testing was carried out on eight strains of yeasts and filamentous fungi: Candida albicans, Candida tropicalis, Candida krusei, Candida glabrata, Trichosporon beigelii, Aspergillus fumigatus, Absidia corymbifera, Trichophyton mentagrophytes. Total were tested 49 substances, which are divided into four groups according to their chemical structure. Derivatives pyrazin-2-carboxamide, 3-derivatives hydrazinopyrazin-2- carboxamide, 2-derivatives isonikotinoylhydrazinkarboxylate, benzoxazin derivatives. Group of derivatives pyrazin-2-carboxamide, which is substituted by low alkyl (-CH3) in the position 5 of pyrazin, and in which is nitrogen of amid group subtituted by the phenyl with electronegative group (-CF3,-F,-Cl,-OH) or with hydroxy group in position m, showed higher..
