The main aim of the methodology is sterilization of skin surface and wounds in veterinary medicine based on low-temperature plasma. Low-temperature plasma has a non-specific antimicrobial effect and therefore can be safely applied to the living tissue without causing any damage. The methodology describes a solution for sterilization of tissues and wounds with bacterial, fungal or yeast contamination. The method can also be used for antimicrobial decontamination as well as for healing of chronic and non-healing wounds. Methodology presents solutions sterilization of tissues and wounds with potential bacterial, fungal or yeast contamination. The method can also be used for antimicrobial decontamination and promote healing of chronic and non-healing wounds