Chemin, Arsene

Elsdon, Emma

Galan, Carmen

Henderson, Alexander S J

Marroux, Hugo

Oliver, Thomas A A

Orr-Ewing, Andrew

Roberts, Gareth

Roettger, Katharina

Street, Steve

Transient electronic and vibrational absorption spectroscopies have been used to investigate whether UV-induced electron-driven proton transfer (EDPT) mechanisms are active in a chemically modified adenine-thymine (A•T) DNA base pair. To enhance the fraction of biologically relevant Watson-Crick (WC) hydrogen-bonding motifs, and eliminate undesired Hoogsteen structures, a chemically modified derivative of A was synthesized, 8-(t-butyl)-9-ethyl-adenine (8tBA). Equimolar solutions of 8tBA and silyl-protected T nucleosides in chloroform yield a mixture of WC pairs, reverse WC pairs and residual monomers. Unlike previous transient absorption studies of WC guanine-cytosine (G•C) pairs, no clear spectroscopic or kinetic evidence was identified for the participation of EDPT in the excited state relaxation dynamics of 8tBA•T pairs, although ultrafast (sub-100 fs) EDPT cannot be discounted. Monomer-like dynamics are proposed to dominate in 8tBA•T

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Is UV-Induced Electron-Driven Proton Transfer Active in a Chemically Modified A•T DNA Base Pair?

Katharina Röttger

Hugo J. B. Marroux

Arsène F. M. Chemin

Emma Elsdon

Thomas A. A. Oliver

Steven T. G. Street

Alexander S. Henderson

M. Carmen Galan

Andrew J. Orr-Ewing

Gareth M. Roberts

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The Journal of Physical Chemistry B

Is UV-Induced Electron-Driven Proton Transfer Active in a Chemically Modified A·T DNA Base Pair?

https://research-information.bris.ac.uk/ws/files/109054593/Adenine_Thymine_accepted_manuscript.pdf

Is UV-Induced Electron-Driven Proton Transfer Active in a Chemically Modified A•T DNA Base Pair?

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