This is the published version. Copyright 1997 de Gruyter.To facilitate evolutionary and forensic studies of DNA
polymorphisms on the Y chromosome, we devised a
multiplex amplification procedure for short-tandemrepeat
(STR) loci. Four tetranucleotide STR loci
(DYS19, DYS390, DYS391, and DYS393) were simultaneously
amplified with FAM-labeled primers and
genotypes were determined with an automated DNA
sequencer. We typed 162 males from three U.S. populations
(African-Americans, European-Americans and
Hispanics) and found that the haplotype diversities
range from 0.920 to 0.969. This quadruplex system
provides a facile means of genotyping these Y chromosome
STRs, and should be useful in population
genetic and forensic applications
