Gao, F

Jiang, D

Li, Q

Lian, Q

Tse, HF

Wong, DS

Xu, G

Yu, Z

Zhang, Y

Cell Death Discovery

PubMed

Recent studies have demonstrated that mesenchymal stem cells (MSCs) can donate mitochondria to airway epithelial cells and rescue mitochondrial damage in lung injury. We sought to determine whether MSCs could donate mitochondria and protect against oxidative stress-induced mitochondrial dysfunction in the cornea. Co-culturing of MSCs and corneal epithelial cells (CECs) indicated that the efficiency of mitochondrial transfer from MSCs to CECs was enhanced by Rotenone (Rot)-induced oxidative stress. The efficient mitochondrial transfer was associated with increased formation of tunneling nanotubes (TNTs) between MSCs and CECs, tubular connections that allowed direct intercellular communication. Separation of MSCs and CECs by a transwell culture system revealed no mitochiondrial transfer from MSCs to CECs and mitochondrial function was impaired when CECs were exposed to Rot challenge. CECs with or without mitochondrial transfer from MSCs displayed a distinct survival capacity and mitochondrial oxygen consumption rate. Mechanistically, increased filopodia outgrowth in CECs for TNT formation was associated with oxidative inflammation-activated NF kappa B/TNF alpha ip2 signaling pathways that could be attenuated by reactive oxygen species scavenger N-acetylcysteine (NAC) treatment. Furthermore, MSCs grown on a decellularized porcine corneal scaffold were transplanted onto an alkali-injured eye in a rabbit model. Enhanced corneal wound healing was evident following healthy MSC scaffold transplantation. And transferred mitochondria was detected in corneal epithelium. In conclusion, mitochondrial transfer from MSCs provides novel protection for the cornea against oxidative stress-induced mitochondrial damage. This therapeutic strategy may prove relevant for a broad range of mitochondrial diseases.Shenzhen Technology Project [JCYJ20140828163633995]; Science and   Technology Foundation of Guangdong Province of China [2015B020225001];   National Natural Science Foundation of China [31270967, 31571407]; Hong   Kong Research Grants Council (RGC) General Research Fund (GRF)   [HKU17113816]; HKU small project funding [201409176251, 201409176221,   201311159115]SCI(E)ARTICLExuguofeng@guanhaobio.com; dongboyaa@163.com; qzlian@hku.hk

Jiang, Dan

Gao, Fei

Zhang, Yuelin

Wong, David Sai Hung

Li, Qing

Tse, Hung-fat

Xu, Goufeng

Yu, Zhendong

Lian, Qizhou

Institutional Repository of Peking University

Cell Death and Disease

Mitochondrial transfer of mesenchymal stem cells effectively protects   corneal epithelial cells from mitochondrial damage

JIANG, D

GAO, F

HKU Scholars Hub

Mitochondrial transfer of mesenchymal stem cells effectively protects corneal epithelial cells from mitochondrial damage.

Mitochondrial transfer of mesenchymal stem cells effectively protects corneal epithelial cells from mitochondrial damage

http://hub.hku.hk/bitstream/10722/242805/1/content.pdf

Mitochondrial transfer of mesenchymal stem cells effectively protects corneal epithelial cells from mitochondrial damage

Abstract

Similar works

Full text

Available Versions

Institutional Repository of Peking University

HKU Scholars Hub

HKU Scholars Hub