Optimum Condition for Solid Fermantation of Trichoderma Humatum in Rice Bran in Order to Produce Celulase by Using Response Surface Methodology

Abstract

Optimum condition for solid fermentation of Trichoderma hamatum in rice bran in order to produce cellulase had been studied by using Response Surface methodology (RSM). Fermentation condition variables that observed was water to rice bran ratio, urea and inoculums additions cellulase activity in FPU/gds (gram of dry solid) became the only dependent variable. The result of empirical equation from this study was proven to be valid within water to rice bran ratio 0,5 - 0,7, urea and inoculums addition where 2-3 % and 1-2% of rice bran weight respactively. Maximum cellulase activity that can be obtain within these ranges was 4.99 ± 0.92 FPU/gds