This study aims to determine is there influence between laughter training on reducing stress levels in the elderly in a nursing institution Hargo Dedali. Stress that occurs in the elderly caused by an inability to adapt or adjust themselves to the environment as well as lower the changes that occurred in his life, feeling depressed and lonely, and feeling isolated, removed, and is not required. The research was conducted in elderly in nursing InstitutionHargo Dedali. the number of study subjects 14 people, consisting of a control group of 7 people and 7 experimental groups. Scale data collection tool in the form of stress levels in the elderly consisting of 59 items. Data analysis was performed with statistical techniques Mann-Whitney U-Test, with the help of statistical program SPSS version 12.0. From the analysis of research data obtained siginifikansi value of 0.04. This suggests that there is influence between laughter training to decrease the stress levels in the elderly in a nursing institution Hargo Dedali