Technique facilitates purification of vaccines and production of immunoadsorption columns exhibiting relatively long stability. Information interests biochemists, medical researchers, and pharmaceutical manufacturers

Smith, D. G.

Tenoso, H. J.

English

NASA Technical Reports Server

1972	 B72-10006 
S NASA TECH BRIEF 
Manned Spacecraft Center	
_ilv 
NASA Tech Briefs announce new technology derived from the U.S. space program. They are issued to encourage 
commercial application. Tech Briefs are available on a subscription basis from the National Technical Information 
Service, Springfield, Virginia 22151. Requests for individual copies or questions relating to the. Tech Brief program may 
be directed to the Technology Utilization Office, NASA, Code KT, Washington, D.C. 20546. 
Covalent Bonding Of Antibodies 
To Polystyrene Latex Beads: A Concept 
A series of chemical reactions boids antibodies to 
one-micron polystyrene latex beads. This technique 
could facilitate the purification of vaccines and the 
production of immunoadsorption columns exhibiting a 
relatively long stability. 
Treatment with glacial acetic acid, fuming red ni-
tric acid, and a solution of sodium hydroxide and so-
dium dithionate, in that order, converts the polystyrene 
• beads into polyaminestyrene beads. Treatment with so-
dium nitrite and hydrochloric acid diazotizes the beads. 
The antibodies are added and the solution neutralized 
with sodium hydroxide. The tyrosine component of the 
antibody molecule forms covalent bonds with diazo 
groups on the bead surface bonding the antibody to the 
polystyrene. 
The physical integrity of the beads is maintained, 
and the utility of the polystyrene beads, both inside and 
outside of a physiological system, is assured. The anti-
bodies can be bound to the polystyrene surface without 
going through the time consuming procedure of dissolving 
the polystyrene, attaching the antibodies, and reforming 
the polystyrene.
This information may be of interest to biochem-
ists, medical researchers, and pharmaceutical manufac-
turers. 
Notes: 
1. This invention is in the conceptual stage only. 
2. Requests for further information may be directed to: 
Technology Utilization Officer 
Manned Spacecraft Center, Code JM7 
Houston, Texas 77058 
Reference: TSP72-1 0006 
Patent status: 
No patent action is contemplated by NASA. 
Source: H. J. Tenoso and D. G. Smith
Aerojet-General
under contract to

Manned Spacecraft Center 
(MSC-13906) 
Category 05 
This document was prepared under the sponsorship of the National 	 Governmtht assumes any liability resulting from the use of the 
Aeronautics and Space Administration. Neither the United States 	 information contained in this document, or warrants that such use 
Government nor any person acting on behalf of the United States 	 will be free from privately ownedrights.
https://ntrs.nasa.gov/search.jsp?R=19720000006 2020-03-17T03:21:00+00:00Z


Covalent bonding of antibodies of polystyrene latex beads: A concept

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