: Sedentary lifestyle according to World Health Organization (WHO) has been identified as the fourth biggest leading cause of death in the world in which already caused approximately 3,2 millions death globaly. Physical exerciseis defined as any body movement produced by the contraction of skeletal muscles that result in a substantial increase over resting energy expenditure. In muscle contraction, calcium is an essential substance for the muscle tissue to contract, so a maintaned calcium level is required in order for the process to be successfully achieved. The goal of this study is to know if there is a difference between blood serum calcium levelbefore and after moderate intensity physical exercise. This study was a pre-experimental study with one group pretest-posttest design approach and purposive sampling method was used to collect the samples. Thirty male and female medical students were evaluated to determine the blood serum calcium level. The mean blood serum calcium level before the physical activity were 9,227 mg/dL and the mean blood serum calcium level after the physical activity were 9,140 mg/dL. Based on Wilcoxon matched-pairs test, the correlation between physical activity and blood serum calcium were not significant (p=0.206). The result of the study suggests that there is no difference of the blood serum calcium before and after moderate intensity physical exercise