The aimed of the research to know the influence of photoperiod of rubber tree (Hevea Brasiliensis Muell. Arg.) in the different medium composition. The research was carried out in the Microcutting Laboratory, PT. Perkebunan Nusantara III Kebun Gunung Pamela Tebing Tinggi, Sumatera Utara, Indonesia. The research began from March to July 2015. The research used completely randomized design with eighteen treathments and six replications. The results showed that interaction of photoperiod and medium with combination of growth regulators were significantly to percent of shoots. Photoperiod 24 h light and the medium of WPM + BAP 0,5 mg/l was the best medium to multiplication of rubber