: House Dust Mites located throughout the world, including Indonesia. Arthropods live in carpets, mattresses, clothes, etc. House Dust Mite populations most found in the bedroom of dust, especially in the mattress dust. House Dust Mites can also be found outside the home as in the nests of bird, mammals, skin surface and other animals. Factors supporting the number of House Dust Mites include temperature and humidity. The purpose of this study to determine the species and density of House Dust Mites in Bitung Karang Ria village, sub-district of Tuminting Manado city. This research method is descriptive survey with a cross sectional approach (cross-sectional). The results of this study suggest that the species of House Dust Mites located in Bitung Karang Ria village is Acarus spp, Tarsonemus spp, Glycyphagus destructor, Dermatophagoides spp, Cheyletus spp. The family room there is a lot more positive while the House Dust Mites populations more in the bedroom. House dust mites average density in the bedroom 2.63 and the family room 2.28. Conclusions: Species of House Dust Mites are most commonly found are Acarus spp and House Dust Mite density was higher in the bedroom than the living room