Sample capsule for determination of oxygen in hemolyzed blood consists of a measured section of polytetrafluoroethylene tubing equipped at each end with a connector and a stopcock valve. This method eliminates errors from air entrainment or from the use of mercury or syringe lubricant

Malik, W. M.

English

NASA Technical Reports Server

October 1967	 Brief 67-10408 
NASA TECH BRIEF 
NASA Tech Briefs are issued to summarize specific innovations derived from the U.S. space program, 
to encourage their commercial application. Copies are available to the public at 15 cents each 
from the Clearinghouse for Federal Scientific and Technical Information, Springfield, Virginia 22151. 
Improved Sample Capsule for Determination of

Oxygen in Hemolyzed Blood 
Measurements of oxygen saturation in hemolyzed 
bloo, based upon the spectrophotometric method 
described in Tech Brief B67-10252, have inherent 
limitations in attainable accuracy, particularly at low 
levels of oxygen saturation, which arise as a con-
sequence of the operations involved in sample prepa-
ration. A sample capsule was designed to eliminate 
errors arising from air entrainment which may occur 
with standard glass syringes, both during sample 
withdrawal and during the introduction of a hemolyz-
ing agent. The new sample capsule obviates the need 
for the addition of mercury for mixing and the need 
for syringe lubricant, both of which are potential 
sources of sample contamination. 
The sample capsule consists of a measured section 
of pzilytetrafluoroethylene tubing equipped at each 
end with a connector and a stopcock valve. Hemolysis 
is accomplished by injection into the capsule of a buf-
fered hemolyzing agent with a 5-inch No. 20 needle 
through a capsule end valve shielded with a 1-inch 
No. 15 needle. Mixing is accomplished by means of a 
0.125-inch brass cube housed in the capsule. Delivery 
of hemolyzed blood into 0.1-mm spectrophotometric
cuvette cells is accomplished through a No. 20 needle-
equipped end valve by a screw-driven pressure plate. 
Notes: 
1. The capsule is designed for use in closed circulating 
systems and is unsuitable for general purpose blood 
sampling from individual subjects. 
2. Inquiries concerning the design and use of the 
sample capsule may be directed to: 
Technology Utilization Officer 
Manned Spacecraft Center 
Houston, Texas 77058 
Reference: B67-10408 
Patent status: 
Inquiries about obtaining rights for the commercial 
use of this invention may be made to NASA, Code 
GP, Washington, D.C. 20546.
Source: W. M. Malik 
of the Institute of Medical Sciences 
at the Presbyterian Medical Center 
under contract to

Manned Spacecraft Center
(MSC-1 1017) 
Category 04 
This document was prepared under the sponsorship of the National	 Government assumes any liability resulting from the use of the 
Aeronautics and Space Administration. Neither the United States 	 information contained in this document, or warrants that such use 
Government nor any person acting on behalf of the United States 	 will be free from privately owned rights.
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Improved sample capsule for determination of oxygen in hemolyzed blood

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