: At an altitude above sea level, the barometric pressure decreases followed by a decrease in air pressure of O2. If someone stays in a highland for several days, weeks, or years, he/she will become acclimatized. One of the main principles of acclimatization is to increase lung ventilation large enough. This study aimed to determinate the vital lung capacity value of students who lived in highlands and of students who lived in lowlands. This study was conducted in two places: SMA 1 Negeri Tomohon and SMA Negeri 1 Manado in December 2015-February 2016. Samples were 40 students: 20 students of SMA Negeri 1 Tomohon and 20 students of SMA Negeri 1 Manado. This was an analytical study with a cross sectional design. The independent T test showed that there was no significant difference between Lung Vital Capacity of students in the highland and students in the lowland with a p value 0.2105. Conclusion: There was no significant difference between Lung Vital Capacity of the students in the highland with students in the lowland