Radiation effect on single cells and cell populations of Chinese hamster lung tissue is studied in vitro. The rate and position as the cell progresses through the generation cycle shows division delay, changes in some biochemical processes in the cell, chromosomal changes, colony size changes, and loss of reproductive capacity

Sinclair, W. K.

English

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August 1968	 Brief 68-10294 
•	 AEC—NASA TECH BRIEF	 ^ WV 
its
AEC-NASA Tech Briefs describe innovations resulting from the research and development program 
of the U.S. AEC or from AEC-NASA interagency efforts. They are issued to encourage commercial 
application. Tech Briefs are published by NASA and may be purchased, at 15 cents each, from 
the Clearinghouse for Federal Scientific and Technical Information, Springfield, Virginia 22151. 
Study of Radiation Effects on Mammalian Cells In Vitro 
.
A study has been conducted to determine the radia-
tion responses of single cells and cell populations 
in vitro. The cells studied were riondifferentiating 
diploid cells, derived from Chinese hamster lung 
tissue,which survive and grow independently under 
constant culture conditions. The radiation used was 
X-rays. 
The study was concerned with those differences 
between cells which affect their radiation response. 
The spec ific variations studied for response charac-
teristics were the rate at which cells progress through 
the generation cycle, and the cell's position within the 
generation cycle. The position of the cells was de-
noted as "age." 
Age response information is important, not only to 
relate these responses to the fundamental physio-
logical state and nature of the cell but also to under-
stand the post-irradiation kinetics of complex popula-
tions both in vitro and in vivo. The age dependence of 
the following responses was discussed: division delay, 
changes in some biochemical processes in the cell, 
chromosome changes, colony size changes, and loss of 
reproductive capacity. The age responses were classi-
fied into two categories: In category I, the responses 
were greatest in the mitotic or in the post-DNA 
synthetic period (G2); they were less in the pre-bNA 
period (G), decreasing to a least value in the DNA 
period itself (S). This category included cell killing, 
total chromosome changes, and colony size changes. 
In category II, responses rise steadily through the 
early part of the cycle, reaching a maximum during 
S and decreasing during G2. This category included 
processes such as DNA synthesis and division delay.
In addition to single cells, cell populations were 
studied. Since the radiation effects of cells are age 
dependent, the effectiveness of a given irradiation 
upon cell populations will depend on the distribution 
of cell ages in that population. The preliminary find-
ings of the characteristics of the surviving population 
of Chinese hamster cells after a dose of 710 rads are 
discussed. 
Notes: 
1. The study has been reported in "Radiation Effects 
on Mammalian Cell Population In Vitro," by 
by Warren K. Sinclair in Radiation Research, 1966; 
North Holland Publishing Company, Amsterdam 
(1967), p.607-631. 
2. Inquiries concerning this study may be directed 
to:
Office of Industrial Cooperation 
Argonne National Laboratory 
9700 South Cass Avenue 
Argonne, Illinois 60439 
Reference: B68-10294 
Source: W. K. Sinclair 
Biological and Medical Research Division 
(ARG-10191) 
Patent status: 
Inquiries about obtaining rights for commercial 
use of this innovation may be made to: 
Mr. George H. Lee, Chief 
Chicago Patent Group 
U.S. Atomic Energy Commission 
Chicago Operations Office 
9800 South Cass Avenue 
Argonne, Illinois 60439
Category 02 
This document was prepared under the sponsorship of the Atomic 	 liability resulting from the use of the information contained in this 
Energy Commission and/or the National Aeronautics and Space document, or warrants that the use of any information, apparatus, 
Administration. Neither the United States Government nor any per- 	 method, or process disclosed in this document may not infringe pri-
son acting on behalf of the United States Government assumes any vately owned rights.
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Study of radiation effects on mammalian cells in vitro

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