: Diarrhea is one of the most frequent diseases of the babies and children in the world. many efforts were made to improve the absorption function of the small intestine, one of the providing of a combination of probiotics (live microorganisms) and prebiotics (substrate), which is known as synbiotic, synbiotic in vivo studies are still very few, especially its handling of non-infectious acute diarrhea is still not yet. This study was aimed to investigate the effect of synbiotic for acute diarrhea in children. This is an a randomized clinical trial double blind in 40 children aged 1-14 years in department of Pediatric, Prof. dr. R. D. Kandou Manado General Hospital. Subjects were randomly allocated into 2 groups, synbiotic and control group. Based on a sample of 40 children study. More men (62.5%) than women (37.5%). Age group 1-5 years is the largest age group with 22 patients. There were no significant differences between treatment groups (sinbiotik) 2.9 days (SB 0.64) and controls 3.2 days (SB 0.61) p = 0.138. conclusion is There is no effect of synbiotic in shortening long of acute diarrhea in children who were treated in the department of Prof. dr. R.D Kandou Manado general hospital