Low back pain is a very common problem and lumbar segmental instability is one of the causes. It is essential to investigate lumbar spine movement in order to understand instability better and as an aid to diagnosis. Digital videofluoroscopy (DVF) provides a method of quantifying the motion of individual vertebra. In this paper, we apply a new version of the Hough transform (HT) to locate the lumbar vertebra automatically in DVF image sequences. At present, this algorithm has been applied to a calibration model and to the vertebra L3 in DVF images, and has shown to provide satisfactory results. Further work will concentrate on reducing the computational time for realtime application, on developing a spatiotemporal sequences method and on determining the spinal kinematics based on the extracted parameters