The focus of this paper is traditional knowledge (TK) and indigenous biological resources protection in South Africa, through the analysis of the existing policies and legislations, in order to provide a useful insight for a developed country such as Japan which has recently adopted the guidelines for the protection of TK and biological resources and promotion of access and benefit sharing (ABS). South Africa is the 3rd most diverse country in terms of natural resources, culture and traditions, languages and geology and its comprehensive legislative framework system shows the country\u27s seriousness to safeguard TK and conserve biological resources for future generations. The paper uses the South Africa\u27s government owned research and technology development institution, Council for Scientific and Industrial Research (CSIR), as an example to demonstrate the application of the TK protection and biodiversity conservation (including access and benefit sharing) laws, through case studies approach for lessons learned for other African countries, contemplating creation of their own TK protection and environmental conservation. Due to the repositioning of CSIR within the local and global research and develop, the organisation has adopted Industrialisation Strategy, and TK will play a significant role in technology development and new business models in rural agroprocessing and production to enhance inclusive development (through benefit sharing) and support economic growth. The paper concludes that TK and indigenous biological resources protection through the relevant government laws, as well as value addition to TK and biodiversity through research and development supported by government funding, is necessary for socioeconomic attainment, especially for local and indigenous communities and rural agroprocessing businesses as part of benefit sharing
