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应用BIGIS-4测序系统完成Glaciecola mesophila sp.nov.的全基因组测序和组装
Authors
于丹
于军
+12 more
任鲁风
俞勇
俞育德
刘贵明
周晓光
李运涛
楚亚男
滕明静
王亮
王绪敏
袁丽娜
韩伟静
Publication date
1 January 2011
Publisher
Abstract
BIGIS-4是中国新一代基于焦磷酸测序技术的测序仪.本实验利用BIGIS-4完成了对Glacielola mesophila sp.nov.(Gmn)的全基因组测序.Gmn是一株从海洋无脊椎动物体内分离到的革兰氏阴性菌.BIGIS-4测序得到152043个高质量的测序片段,平均读长406bp.测序片段由BIGIS-4系统后处理模块组装.除单核苷酸同聚体引起的测序错误外,测序结果中没有检测到其他低质量错误.Gmn基因组全长5144318bp,共注释得到4303个基因,其中有大量的代谢基因,与菌种在海洋表面非脊椎动物的生长环境相关.Gmn的冷适应和信号转导相关基因为其对海洋低温环境的适应提供了依据
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Last time updated on 29/11/2016