Frequency of Prevalence of Klebsiella pneumoniae in Clinical Samples and the Evaluation of the Role of Efflux Pump in Determining Antibiotic Resistance
In this study 193 Klebsiella pneumoniae were isolated from urinary tract infections, ulcers,
sputum and blood. Initially, Mac agar medium was used to isolate the bacterium, and for
each suspected isolate, pink and aqueous colonies were stained and biochemical tests of
catalase, oxidase, TSI, IMVIC Test and urase were performed. Confirmation of the isolates
using 16SrRNA sequencing Some isolates are evaluated. Then all isolates evaluated for
sensitivity to antibiotics such as Ampicillin, Amoxicillin clavulanate, Piperacillin,
Cefoxitin, Cefuroxime Imipenem, Tetracyclines, Nitrofurantoin, Polymyxin B Colistin,
they use disk diffusion test. In the process, the presence of the acr efflux pump gene is
confirmed by using an specific primer namely Acr primer, and finally, using phenylalaninearginine-
beta naphthylamide inhibitor, the relationship between antibiotic resistance and
efflux pump function is evaluated. Overall, 50.2% of the collected samples contained
Klebsiella. Thus, 193 of 384 clinical specimens contained Klebsiella. Of the 193 positive
samples, the groin lesions had the highest percentage and the abscess had lowest percentage
of Klebsiella infection, although Klebsiella was significantly separated from the throat,
sputum, catheter and foley. Antibiotics, cefazolin, ceftazidime, ciprofloxacin,
Chloramphenicol, tetracycline had higher antibacterial activity. Results were analyzed by
Whonet 5 and SPSS software
