SKIN STRIPPING AS A POTENTIAL METHOD TO DETERMINE INVIVO CUTANEOUS METABOLISM OF TOPICALLY APPLIED DRUGS

Abstract

By chromatographinganextractof thetapesobtainedin a skinstrippingprocedure,cutaneousmetabolismofcompoundsaftertopicaladministrationmaybeobservable,providedthatoutwardtransdermalmigrationoccurs.This methodmaybe helpful,especiallyin situationswherenodifferentiationbetweencutaneousandsystemicmetabolismcanbemadedueto theexperimentaldesignor theverylowsystemicconcentra-tions.Throughuseof thismethodology,it canbeassessedthat the penetrationenhancerfor percutaneousabsorption,Azone©, isonlypresentastheparentcompoundin thestratumcorneum,whereastheanti-acneagentCyoctolundergoescutaneousbiotransformationduringskinpassag