Small peptide tags such as c-myc, HA, or FLAG tag have facilitated efficient Westernblotting
of proteins of interest especially when specific antibodies for the proteins are
not available. However, the conventional Western-blotting requires the multi-steps
process taking at least several hours up to two days. With examples of various applications,
here we show a convenient and time-saving method for protein detection which
employs a fluorescent chemical BDED and its binding peptide RC-tag. And we propose
“Estern staining”, as a standard term for protein detection method using fluorescent
chemicals and their binding small peptide tags. Eastern staining may substitutes for the
time-consuming “immuno-staining” in many versatile applications.22Yothe
