In the present study, three strains of Candida glabrata have been investigated to shed light on the mechanisms

involved in azole resistance during adherence and biofilm formation. In particular, a clinical isolate, susceptible to

azole-based drugs, DSY562 and two different resistant mutagenic strains deriving from DSY562, SFY114 and

SFY115, have been analysed with different approaches for their cell wall composition and properties. A proteomic

analysis revealed that the expression of six cell wall-related proteins and biofilm formation varied between the

strains. The SFY114 and SFY115 strains resulted to be less hydrophobic than the susceptible parental counterpart

DSY562, on the other hand they showed a higher amount in total cell wall polysaccharides fraction in the total

cell wall. Accordingly to the results obtained from the hydrophobicity and adherence assays, in the resistant strain

SFY115 the biofilm formation decreased compared to the parental strain DSY562. Finally, the total glucose amount

in resistant SFY115 was about halved in comparison to other strains. Taken together all these data suggest that

azole drugs may affect the cell wall composition of C. glabrata, in relation to the different pathogenic behaviours

Vitali, Alberto

Vavala, Elisabetta

Marzano, Valeria

Leone, Claudia

Castagnola, Massimo

Iavarone, Federica

Angiolella, Letizia

English

Alberto Vitali

Elisabetta Vavala

Valeria Marzano

Claudia Leone

Massimo Castagnola

Federica Iavarone

Letizia Angiolella

Crossref

Journal of Chemotherapy

Cell wall composition and biofilm formation of azoles-susceptible and -resistant <i>Candida glabrata</i> strains

Over decadal-centennial timescales, only a few mechanisms in the carbon-climate system could cause a massive net redistribution of carbon from land and ocean systems to the atmosphere in response to climate warming. The largest such climate-vulnerable carbon pool is the old organic carbon (OC) stored in Arctic permafrost (perennially frozen) soils. Climate warming, both predicted and now observed to be the strongest globally in the Eurasian Arctic and Alaska, causes thaw-release of old permafrost carbon from local tundra sites. However, a central challenge for the assessment of the general vulnerability of this old OC pool is to deduce any signal integrating its release over larger scales. Here we examine radiocarbon measurements of molecular soil markers exported by the five Great Russian-Arctic Rivers (Ob, Yenisey, Lena, Indigirka and Kolyma), employed as natural integrators of carbon release processes in their watersheds. The signals held in estuarine surface sediments revealed that average radiocarbon ages of ii-alkanes increased east-to-west from 6400 yr BP in Kolyma to 11 400 yr BP in Ob. This is consistent with westwards trends of both warmer climate and more degraded organic matter as indicated by the ratio of high molecular weight (HMW) n-alkanoic acids to HMW n-alkanes. The dynamics of Siberian permafrost can thus be probed via the molecular-radiocarbon signal as carried by Arctic rivers. Old permafrost carbon is at present vulnerable to mobilization over continental scales. Climate-induced changes in the radiocarbon fingerprint of released permafrost carbon will likely depend on changes in both permafrost coverage and Arctic soil hydraulics. © 2011 Author(s)

Gustafsson, Ö

Van Dongen, B. E.

Vonk, J. E.

Dudarev, O. V.

Semiletov, I. P.

The University of Manchester - Institutional Repository

Widespread release of old carbon across the Siberian Arctic echoed by its large rivers

Abstract. Over decadal-centennial timescales, only a few mechanisms in the carbon-climate system could cause a massive net redistribution of carbon from land and ocean systems to the atmosphere in response to climate warming. The largest such climate-vulnerable carbon pool is the old organic carbon (OC) stored in Arctic permafrost (perennially frozen) soils. Climate warming, both predicted and now observed to be the strongest globally in the Eurasian Arctic and Alaska, caused thaw-release of old permafrost carbon from local tundra sites. However, a central challenge for the assessment of the general vulnerability of this old OC pool is to deduce any signal integrating its release over larger scales. Here we examine radiocarbon measurements of molecular soil markers exported by the five Great Russian-Arctic Rivers (Ob, Yenisey, Lena, Indigirka and Kolyma), employed as natural integrators of carbon release processes in their watersheds. Average radiocarbon ages of n-alkanes increased east-to-west from 6400 yr BP in Kolyma to 11 400 yr BP in Ob, consistent with a warmer climate and more degraded organic matter westward. The dynamics of Siberian permafrost can thus be probed via radiocarbon river signals. Old permafrost carbon is at present vulnerable to mobilization over continental scales. Climate-induced changes in the radiocarbon fingerprint of released permafrost carbon will likely depend on changes in both permafrost coverage and Arctic soil hydraulics.
                        </jats:p

Ö. Gustafsson

B. E. van Dongen

J. E. Vonk

O. V. Dudarev

I. P. Semiletov

In the present study, three strains of Candida glabrata have been investigated to shed light on the mechanisms
involved in azole resistance during adherence and biofilm formation. In particular, a clinical isolate, susceptible to
azole-based drugs, DSY562 and two different resistant mutagenic strains deriving from DSY562, SFY114 and
SFY115, have been analysed with different approaches for their cell wall composition and properties. A proteomic
analysis revealed that the expression of six cell wall-related proteins and biofilm formation varied between the
strains. The SFY114 and SFY115 strains resulted to be less hydrophobic than the susceptible parental counterpart
DSY562, on the other hand they showed a higher amount in total cell wall polysaccharides fraction in the total
cell wall. Accordingly to the results obtained from the hydrophobicity and adherence assays, in the resistant strain
SFY115 the biofilm formation decreased compared to the parental strain DSY562. Finally, the total glucose amount
in resistant SFY115 was about halved in comparison to other strains. Taken together all these data suggest that
azole drugs may affect the cell wall composition of C. glabrata, in relation to the different pathogenic behaviours

VAVALA, ELISABETTA

LEONE, CLAUDIA

ANGIOLELLA, Letizia

Archivio della ricerca- Università di Roma La Sapienza

Cell wall composition and biofilm formation of azoles-susceptible and -resistant Candida glabrata strains

Over decadal-centennial timescales, only a few mechanisms in the carbon-climate system could cause a massive net redistribution of carbon from land and ocean systems to the atmosphere in response to climate warming. The largest such climate-vulnerable carbon pool is the old organic carbon (OC) stored in Arctic permafrost (perennially frozen) soils. Climate warming, both predicted and now observed to be the strongest globally in the Eurasian Arctic and Alaska, causes thaw-release of old permafrost carbon from local tundra sites. However, a central challenge for the assessment of the general vulnerability of this old OC pool is to deduce any signal integrating its release over larger scales. Here we examine radiocarbon measurements of molecular soil markers exported by the five Great Russian-Arctic Rivers (Ob. Yenisey, Lena, Indigirka and Kolyma), employed as natural integrators of carbon release processes in their watersheds. The signals held in estuarine surface sediments revealed that average radiocarbon ages of n-alkanes increased east-to-west from 6400 yr BP in Kolyma to 11 400 yr BP in Ob. This is consistent with westwards trends of both warmer climate and more degraded organic matter as indicated by the ratio of high molecular weight (HMW) n-alkanoic acids to HMW n-alkanes. The dynamics of Siberian permafrost can thus be probed via the molecular-radiocarbon signal as carried by Arctic rivers. Old permafrost carbon is at present vulnerable to mobilization over continental scales. Climate-induced changes in the radiocarbon fingerprint of released permafrost carbon will likely depend on changes in both permafrost coverage and Arctic soil hydraulics.ISSN:1726-4170ISSN:1726-417

Gustafsson, Örjan

van Dongen, Bart

Vonk, Jorien

Dudarev, Oleg V.

Semiletov, Igor P.

Repository for Publications and Research Data

Over decadal-centennial timescales, only a few mechanisms in the carbon-climate system could cause a massive net redistribution of carbon from land and ocean systems to the atmosphere in response to climate warming. The largest such climate-vulnerable carbon pool is the old organic carbon (OC) stored in Arctic permafrost (perennially frozen) soils. Climate warming, both predicted and now observed to be the strongest globally in the Eurasian Arctic and Alaska, causes thaw-release of old permafrost carbon from local tundra sites. However, a central challenge for the assessment of the general vulnerability of this old OC pool is to deduce any signal integrating its release over larger scales. Here we examine radiocarbon measurements of molecular soil markers exported by the five Great Russian-Arctic Rivers (Ob, Yenisey, Lena, Indigirka and Kolyma), employed as natural integrators of carbon release processes in their watersheds. The signals held in estuarine surface sediments revealed that average radiocarbon ages of ii-alkanes increased east-to-west from 6400 yr BP in Kolyma to 11 400 yr BP in Ob. This is consistent with westwards trends of both warmer climate and more degraded organic matter as indicated by the ratio of high molecular weight (HMW) n-alkanoic acids to HMW n-alkanes. The dynamics of Siberian permafrost can thus be probed via the molecular-radiocarbon signal as carried by Arctic rivers. Old permafrost carbon is at present vulnerable to mobilization over continental scales. Climate-induced changes in the radiocarbon fingerprint of released permafrost carbon will likely depend on changes in both permafrost coverage and Arctic soil hydraulics

NARCIS 

Over decadal-centennial timescales, only a few mechanisms in the carbon-climate system could cause a massive net redistribution of carbon from land and ocean systems to the atmosphere in response to climate warming. The largest such climate-vulnerable carbon pool is the old organic carbon (OC) stored in Arctic permafrost (perennially frozen) soils. Climate warming, both predicted and now observed to be the strongest globally in the Eurasian Arctic and Alaska, causes thaw-release of old permafrost carbon from local tundra sites. However, a central challenge for the assessment of the general vulnerability of this old OC pool is to deduce any signal integrating its release over larger scales. Here we examine radiocarbon measurements of molecular soil markers exported by the five Great Russian-Arctic Rivers (Ob, Yenisey, Lena, Indigirka and Kolyma), employed as natural integrators of carbon release processes in their watersheds. The signals held in estuarine surface sediments revealed that average radiocarbon ages of &lt;i&gt;n&lt;/i&gt;-alkanes increased east-to-west from 6400 yr BP in Kolyma to 11 400 yr BP in Ob. This is consistent with westwards trends of both warmer climate and more degraded organic matter as indicated by the ratio of high molecular weight (HMW) &lt;i&gt;n&lt;/i&gt;-alkanoic acids to HMW &lt;i&gt;n&lt;/i&gt;-alkanes. The dynamics of Siberian permafrost can thus be probed via the molecular-radiocarbon signal as carried by Arctic rivers. Old permafrost carbon is at present vulnerable to mobilization over continental scales. Climate-induced changes in the radiocarbon fingerprint of released permafrost carbon will likely depend on changes in both permafrost coverage and Arctic soil hydraulics

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Biogeosciences

http://hdl.handle.net/1871.1/6792d7dd-18e9-44c9-90ba-d8babf8c4a40

Cell wall composition and biofilm formation of azoles-susceptible and -resistant Candida glabrata strains

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The University of Manchester - Institutional Repository

Crossref

Archivio della ricerca- Università di Roma La Sapienza

Repository for Publications and Research Data

The University of Manchester - Institutional Repository

NARCIS

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