Briers, Yves

Desmet, Tom

João de Carvalho Maurício da Fonseca, Maria

Stals, Ingeborg

Van Landschoot, Anita

English

de Carvalho Maurício da Fonseca, Maria João

Ghent University Academic Bibliography

Engineering techniques for modular proteins Library of multicatalytic enzymatic complexes based on dockerin-cohesin affinity active on arabinoxylan   high-throughput Activity test (96-well plate) Enzymatic reactions with appropriate substrates     high-throughput Analysis of AXOS formed By DSA-FACE: DNA sequencer-aided fluorophore-assisted carbohydrate electrophoresis  high-throughput       1. Why? Arabinoxylan-oligosaccharides are potential PREBIOTICS that can improve the overall intestinal health.  Processing of residual biomass as raw materials provides economic and ecological benefits due to its biorenewability. Florestal, agricultural and industrial wastes or by-products contain considerable amounts of arabinoxylan Arabinoxylans are complex substrates and its hydrolysis require the combination of diverse enzymatic activities. 3. Experimental set-up ENZYMATIC DEGRADATION OF ARABINOXYLAN:  combination of different substrate specificities            Headed by Professor Yves Briers Yves.Briers@UGent.be Focuses on synthetic biology of modular proteins • Modular proteins: each module folds autonomously and has a dedicated function Design and development of tailor-made modular proteins for customized applications in medical and industrial biotechnology. • Engineering of the specific protein modular composition  Novel engineering and selection methods are developed to pursue these goals PhD students & Research Agnieszka Łątka Agnieszka.Latka@ugent.be ‘I am trying to figure out how single phage proteins influence host specificity of whole bacteriophages.’   Hans Gerstmans hans.gerstmans@ugent.be ‘My research is about developing high-throughput screening assays for modular enzymes.’   Julie Vanderstraeten julie.vanderstraeten@ugent.be ‘I am developing designer cellulosomes to efficiently convert waste material into second generation sugars and other valuable end products.’   Maria Fonseca MariaJoao.Fonseca@ugent.be ‘I aim to develop enzymatic complexes for the high-throughput production of arabinoxylan-oligosaccharides (AXOS) originated from waste material.’   Silke Vlyminck Silke.Vlyminck@ugent.be ‘I am developing new enzymatic treatment methods for fungal infections.’  Laboratory of Applied Biotechnology (L.A.B.) AXH-d3  α-L-arabinofuranosidases AXH-m2,3 α-L-arabinofuranosidases       4. In the L.A.B. …  A. Looking for suitable multicatalytic complexes depending on the AXOS needed. Enzymatic reactions:  96 reactions with  different combinations  of dockerins, cohesins  and enzymes. Enzymatic activities                             … Dockerins                 … Cohesins                   … B. DSA-FACE (fluorescent detection) 2. How? HIGH-THROUGHPUT CUSTOMIZED PRODUCTION OF ARABINOXYLAN-OLIGOSACCHARIDES (AXOS) Maria João Maurício da Fonseca1, Ingeborg Stals2, Tom Desmet3, Anita Van Landschoot1, Yves Briers1* 1Department of Applied Biosciences, Ghent University, Ghent, Belgium, 2Department of Materials, Textiles and Chemical Engineering, Ghent University, Ghent, Belgium, 3Department of Biochemical and Microbial Technology, Ghent University, Ghent, Belgium      4th European Summer School on Industrial Biotechnology                        Food and Biorefinery Enzymology                                                    25-29 September 2017               Exo-1,4-β-xylosidases GH10 endo-1,4-β-xylanases GH11 endo-1,4-β-xylanases xylose units XA2XX active AXH-d3 Relative fluorescent units (RFU) Resolution good separation for AXOS LOD from 38 pM to 55 pM for the AXOS studied Analysis time 1 plate in approximately 15 h xylose units Relative fluorescent units (RFU) XXXX XA3XX XA2XX active AXH-m2,3 heat  inactivated AXH-d3 DSA-FACE electropherograms: XA2+3XX heat  inactivated AXH-m2,3 1. Reaction with XA2+3XX and  a AXH-d3 enzyme 2. Reaction with XA2XX and  XA3XX and a AXH-m2,3  enzyme   …  

High-throughput customized production of arabinoxylan-oligosaccharides (AXOS)

https://biblio.ugent.be/publication/8533881/file/8533885.pdf

High-throughput customized production of arabinoxylan-oligosaccharides (AXOS)

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Ghent University Academic Bibliography