En la preocupación que ROL demuestra por clarificar los campos de la 'Enfermería', sus especialidades y profesiones paralelas, nos brinda un espacio para presentar la comunicación que sobre 'La profesión de Dietista', expusimos en el 1.er Congreso de la Sociedad Española de Nutrición, celebrado en Galicia en octubre de 1979. La nutrición aplicada, o sea la DIETÉTICA, al igual que las ciencias de la alimentación, relativamente modernas, están en continua evolución. Por esto, no es de extrañar el desconocimiento de la profesión de 'Dietista', que por parte oficial observamos en nuestro país y que nos ha inducido a presentar esta ponencia

Hee Choi (4435078)

Hong Lee (3503117)

Jae Han (3414722)

Kyung Lee (1865221)

Venugopal Nair (222225)

Yongxiu Yao (229112)

Young Park (3745101)

Additional file 3. Sequencing results of TVB#2-transfected DF-1 clones using chromatography. Wild type (WT) DF-1 cells with tvb s3 genotypes were used as the control. The red arrow indicates the guide RNA recognition site, and orange rectangles indicate specific single nucleotide polymorphisms in tvb

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MOESM3 of Acquisition of resistance to avian leukosis virus subgroup B through mutations on tvb cysteine-rich domains in DF-1 chicken fibroblasts

International audienceAbstractAvian leukosis virus (ALV) is a retrovirus that causes tumors in avian species, and its vertical and horizontal transmission in poultry flocks results in enormous economic losses. Despite the discovery of specific host receptors, there have been few reports on the modulation of viral susceptibility via genetic modification. We therefore engineered acquired resistance to ALV subgroup B using CRISPR/Cas9-mediated genome editing technology in DF-1 chicken fibroblasts. Using this method, we efficiently modified the tumor virus locus B (tvb) gene, encoding the TVB receptor, which is essential for ALV subgroup B entry into host cells. By expanding individual DF-1 clones, we established that artificially generated premature stop codons in the cysteine-rich domain (CRD) of TVB receptor confer resistance to ALV subgroup B. Furthermore, we found that a cysteine residue (C80) of CRD2 plays a crucial role in ALV subgroup B entry. These results suggest that CRISPR/Cas9-mediated genome editing can be used to efficiently modify avian cells and establish novel chicken cell lines with resistance to viral infection

Lee, Hong Jo

Lee, Kyung Youn

Park, Young Hyun

Choi, Hee Jung

Yao, Yongxiu

Nair, Venugopal

Han, Jae Yong

Archive Ouverte en Sciences de l'Information et de la Communication

Acquisition of resistance to avian leukosis virus subgroup B through mutations on tvb cysteine-rich domains in DF-1 chicken fibroblasts

Abbreviations 
CRDs: cysteine rich domains; ALV: avian leukosis virus; tvb: tumor virus locus B; tva: tumor virus locus A; tvc: tumor virus locus C; W38: tryptophan 38; CRISPR/ Cas9: clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated; DMEM: Dulbeccos minimum essential medium; FBS: fetal bovine serum; ABAM: antibiotic–antimycotic; WL: White Leghorn; CEF: chicken embryonic fbroblast; EDTA: ethylenediaminetetraacetic acid; dpi: days postinfection; SAS: the statistical analysis system; ANOVA: analysis of variance; WT: wild type; GFP: green fuorescent protein; TNFRSF10B: tumor necrosis factor receptor superfamily member 10B; DD: death domain; PAM: protospacer adjacent motif; LTR: long terminal repeat; RCAS: replication-competent ALV LTR with a splice acceptor; HIV: human immunodefciency virus; EIAV: equine infectious anemia virus; FIV: feline immunodefciency virus; RABS: rabies virus; HVEM: herpes virus entry mediator; LDLR: low-density lipoprotein receptor; PGC: primordial germ cell; gRNA: guide RNA.Abstract
              Avian leukosis virus (ALV) is a retrovirus that causes tumors in avian species, and its vertical and horizontal transmission in poultry flocks results in enormous economic losses. Despite the discovery of specific host receptors, there have been few reports on the modulation of viral susceptibility via genetic modification. We therefore engineered acquired resistance to ALV subgroup B using CRISPR/Cas9-mediated genome editing technology in DF-1 chicken fibroblasts. Using this method, we efficiently modified the tumor virus locus B (tvb) gene, encoding the TVB receptor, which is essential for ALV subgroup B entry into host cells. By expanding individual DF-1 clones, we established that artificially generated premature stop codons in the cysteine-rich domain (CRD) of TVB receptor confer resistance to ALV subgroup B. Furthermore, we found that a cysteine residue (C80) of CRD2 plays a crucial role in ALV subgroup B entry. These results suggest that CRISPR/Cas9-mediated genome editing can be used to efficiently modify avian cells and establish novel chicken cell lines with resistance to viral infection.This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2015R1A3A2033826). Authors also acknowledge the funding support from the Royal Society International Professorships

SNU Open Repository and Archive

Veterinary Research

Cervera, Pilar

Rigolfas, Rita

Candela, M.

Cugat, P.

Diposit Digital de la Universitat de Barcelona

LA PROFESIÓN DE D IETISTA Por P. Cervera; R. Rigolfas; M. Candela; P. Cugat. Grupo de Dietética de Barcelona INTRODUCCIÓN En la preocupación que ROL demuestra por clarificar los campos de la "Enfermería", sus especialidades y profesiones paralelas, nos brinda un espacio para presentar la comunicación que sobre "La profesión de Dietista", expusimos en el 1.er Congreso de la Sociedad Española de Nutrición, celebrado en Galicia en octubre de 1979. La nutrición aplicada, o sea la DIETÉTICA, al igual que las ciencias de la alimentación, relativamente modernas, están en continua evolución. Por esto, no es de extrañar el desconocimiento de la profesión de " Dietista", que por parte oficial observamos en nuestro país y que nos ha inducido a presentar esta ponencia. 26 

La profesión de dietista

Abstract Avian leukosis virus (ALV) is a retrovirus that causes tumors in avian species, and its vertical and horizontal transmission in poultry flocks results in enormous economic losses. Despite the discovery of specific host receptors, there have been few reports on the modulation of viral susceptibility via genetic modification. We therefore engineered acquired resistance to ALV subgroup B using CRISPR/Cas9-mediated genome editing technology in DF-1 chicken fibroblasts. Using this method, we efficiently modified the tumor virus locus B (tvb) gene, encoding the TVB receptor, which is essential for ALV subgroup B entry into host cells. By expanding individual DF-1 clones, we established that artificially generated premature stop codons in the cysteine-rich domain (CRD) of TVB receptor confer resistance to ALV subgroup B. Furthermore, we found that a cysteine residue (C80) of CRD2 plays a crucial role in ALV subgroup B entry. These results suggest that CRISPR/Cas9-mediated genome editing can be used to efficiently modify avian cells and establish novel chicken cell lines with resistance to viral infection

Hong Jo Lee

Kyung Youn Lee

Young Hyun Park

Hee Jung Choi

Yongxiu Yao

Venugopal Nair

Jae Yong Han

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Additional file 1. Establishment of tvb -modified DF-1 clones. (A) DF-1 cell morphology during in vitro culture. Scale bar = 50 Âľm. (B) Establishment of 21 individual DF-1 clones. Wild type (WT) DF-1 cells were used as the control. Scale bar = 200 Âľm

MOESM1 of Acquisition of resistance to avian leukosis virus subgroup B through mutations on tvb cysteine-rich domains in DF-1 chicken fibroblasts

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La profesión de dietista

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Archive Ouverte en Sciences de l'Information et de la Communication

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Diposit Digital de la Universitat de Barcelona

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