The growth of Agrobacterium tumefaciens in nutrient solution can be inhibited by all ᴅ-amino acids used in these experiments. The degree of inhibition is of different in magnitude; in some cases the inhibition is complete. The inhibition is dependent on the concentration of ᴅ-amino acid and can not be counteracted by addition of L-amino acid or glycine; it is increased by K2CO3. After 28 transfers, all ᴅ-amino acid used in these experiments lead to reduction or complete loss of virulence. It can be assumed that ᴅ-amino acids induce independently loss of virulence and inhibition of growth
