CORE
🇺🇦
make metadata, not war
Services
Services overview
Explore all CORE services
Access to raw data
API
Dataset
FastSync
Content discovery
Recommender
Discovery
OAI identifiers
OAI Resolver
Managing content
Dashboard
Bespoke contracts
Consultancy services
Support us
Support us
Membership
Sponsorship
Community governance
Advisory Board
Board of supporters
Research network
About
About us
Our mission
Team
Blog
FAQs
Contact us
Selection of Reference Genes for Toxicological Mechanism Research on Ruditapes phUippinarum Exposed to Ammonia Nitrogen
Authors
丛明
吕家森
+4 more
曹滕飞
李兆艳
李成华
赵建民
Publication date
1 January 2017
Publisher
Abstract
为了准确和系统地分析NH_3-N对菲律宾蛤仔(Ruditapes philippinarum)毒性的分子机制,并对相关基因的转录组学数据进行验证,利用ge Norm和Normfinder这2种软件,以暴露30 d中不同时间点的鳃组织c DNA为模板,从18S r RNA(18S)、beta-actin(Actin)、beta-tubulin(Tubu)、elongation factor 1-alpha(EF1α)、cyclophilin A(Cy PA)、Ubiquitin(Ub)等6个备选管家基因中,筛选可以稳定表达的内参基因,作为转录组学分析的内参基因。以稀释50倍的鳃组织c DNA模板作为检测模板,ge Norm软件分析获得6对备选内参基因的表达稳定性依次为:EF1α〉Cy PA〉Actin〉Tubu〉Ub〉18S,较优内参为EF1α和Cy PA;Norm Finder软件分析获得6对备选内参基因的表达稳定性为:EF1α〉Actin〉Tubu〉Cy PA〉Ub〉18S,最优内参基因为EF1α。为了验证上述筛选结果,分别以EF1α和Cy PA作为内参基因研究unigene1的表达趋势,发现以EF1α为内参基因时,unigene1的q RT-PCR的表达趋势与其转录组表达倍数的变化趋势一致。因此确定NH_3-N暴露菲律宾蛤仔后,鳃组织中表达最为稳定的内参基因为EF1α
Similar works
Full text
Available Versions
Institutional Repository of Yantai Institute of Coastal Zone Research, CAS
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:ir.yic.ac.cn:133337/26427
Last time updated on 24/06/2021