Molecular imprinting is a technique to synthesize molecularly imprinted polymers(MIPs) with specific and selective recognition ability for a given template molecule. As a molecular recognition component, MIPs combined with highly sensitive fluorescent detection to construct molecular imprinting-based fluorescence sensors(MI-FL sensors),which have attracted much attention in the field of trace detection of environmental organic pollutants. According to the expression of fluorescence emission signal, this review introduces the various strategies for MI-FL sensors. The applications of MI-FL sensors in pesticide residues, estrogen, and antibiotic in the environment are described briefly,and the challenges and development are prospected