Renal lymph oxygen tension in dogs during graded renal ischemi

Cockett, A. T. K.

Kado, R. T.

Kazmin, M.

Moore, R. S.

Roberts, R. P.

English

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RENAL LYMPH OXYGEN TENS I ON DURl NG GRADED RENAL I SCHEMI A 
A.T.K. Cockett, H.D., E.?. Kazmin, i4.D., R.S. l.loore, 3.s .  
R. T. Kado, 8.A. and R. P. Roberts, 3.A. 
Concentrations o f  respi ra tory  gases in  t issues such as the kidney are 
important f o r  several reasons. They ind icate the re la t i onsh ip  between 
ox idat ive metabolism o f  renal t issue and i t ' s  blood supply. 
t o  understand the i n te r - re la t i onsh ip  between external respi ra t ion,  blood and 
They help us 
i n te rna l  respi ra t ion.  
Since lymphatics d ra in  the i n t e r s t i t i a l  spaces, we f e l t  t h a t  renal 
lymph f l u i d  would serve as a useful  means o f  assessing t issue oxidat ion.  
Renal capsular lymphatics were cannulated i n  ten anesthetized mongrel 
dogs. Renal lymph was co l lected under mineral o i l ,  An endotrachea 
was connected t o  an automatic respi ra tor .  Compressed a i r  was admin 
t o  seven animals. Oxygen-air (20-800/,) was administered t o  the rema 
tube 
stered 
n ing three. 
Renal ischemia was produced by a Goldblat t  clamp. An electro-magnetic f low- 
meter gauged the  degree o f  renal a r t e r i a l  const r ic t ion,  
The normal renal lymph oxygen tension i s  l i s t e d  i n  tabular form (Table I). 
Mean renal  lymph oxygen tension was 150 rnm o f  H5. 
a r t e r i a l  oxygen tension was 99 mm o f  Hg. (mean). The mean renal venous 
Corresponding femoral 
oxygen tension was 64 mm of Hg. 
From the Department o f  Surgery/Urology, Harbor General Hospi ta l  and 
/ 
t h e  Un ive rs i t y  o f  C a l i f o r n i a  (Los Angeles) School of Medicine. 
U. S. Publ ic  Health Service Grant HE-39834-02, and the National Aeronautics 
and Space Administrat ion rJsG 237-62. Renal ischemia (50%) resul ted i n  a . 
Supported by 
4 
reduct ion i n  lymph oxygen tension. PO2 was now 75-115 mrn o f  Hg. 
Our data would suqqest t h a t  oxvdative metabolic nrocesses are extremely 
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active within the kidney, Renal lymph oxygen tension levels significantly 
exceeded the arterial levels. The relatively high level of oxygen tension 
at the renal interstitial level was completely unexpected. Our findings are 
not in keeping with the levels published by Uergofsky et a1.l who monitored 
thoracic duct lymph concentrations of respiratory gases. Their oxygen ten- 
sion value of 40 mm of Hg. is much lower than our renal lymph levels. 
Further work in this area is clearly indicated. Underway in our labora- 
tory are studies determining lactic acidipyruvic acid concentrations in renal 
lymph fiuid. It would appear that the kidney has a number of  efficient enzy- 
matic processes for storage of oxygen. 
REFEP.ENCE 
1 .  dergofsky, E. H., Jacobson, J.  H. I I  and Fishman, A. P.: The use of 
lymph for the measurement of gas tensions in interstitial fluid and 
tissues. J. Clin. Invest. 1962, 41:1971. 
TABLE I 
NORMAL RENAL LYMPH OXYGEN TENS I ON 
DOG # LYMPH PO ARTERY PO2 RENAL VEIN PO, 
1 158 mm Hg 64 mm Hg 56 mm Hg 
2 133 
142 
166 
3 
4 
5 138 
6 166 
90 
99 
98 
1 46 
100 
59 
76 
57 
MEAN -- 150 mm Hg 99 mm Hg 64 mm Hg 
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Di s t r ibu  t i on 15 .  s t 
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Renal Lymph Oxygen Tension During Graded Renal Ischemia

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