Bacterial contaminant in Biosatellite water syste

Bryce, A. J.

English

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Drin:•iu^ t'!nter for the prirt.ltc vns derivod from the !)ycl	 file1
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contoIiwd a dftvrete umLcr. col l cc. Lioil, I+ u1' Ific;lt,ion and (II p-rising, ny,:tvm• A
portion of 01(: t :•aLcr war also used in the spacceraf t therm: ► ]. colltrol. sk1)l!'-y_ ((*'•'1
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eorvAr:li.nts were applied Lo Lho. hc 1 t.;7.71e WilLer sail+l)]y sy!,t(•nl to filS111T It's
canpatibi.liLy with thw primaLe c::I)arin:;:nL ob .jucti.ves. The cheraical puri;.3zat."oat
Gf t11C W:11C'1' 1::t5 ACC( ,n.pli::llcd by t.l'[.atClCnt Wltll a t;cri.cs of lc)11 exc:hallgo culuo- ► 1:;.
A bacteria l. fill , cr wJr used to rc:n!.wc! bacteria and fungi ori.Lina tA ng fraa ti(c
fuel cell alid ru:iin bads ruspvctivoly. Chemi.cal purification of tho fu(!] cc'l.
elf 111cllt %.'a	 .1('('oalpl.i,;llc ' d t•:i th ic!w IWC)1)lc!1n0. 	 Uf.fec LiVe hic)1o,, ;i.ca1 trc at•rlcnt
hov vcr, t:.1s 11111 h tt•ML (11-fiicult to acco!l!pli.4. A ina::in;l(t:, all.ow11!]u viable
orl, :r► i:;i:	 c olnit 'f'	 20,i cul u ,iic:s lien	 c,f	 J)OL:11)lo	 t,:at • ('r	 V.'Of;	 r 1)( .;	 } ficcl,
The	 maxir.'• • :i a l lownbl y coli form cow.it.	 v— s,	 2 .2	 colon.-Ocs	 pur	 100 mil 1 J 1 i l -!r!:	 of!
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:1. Sysrcm coi, •ponents were t:otuhrd l:i.t-h	 di si.nfc-CU'd,	 i;loved hands. only.
/I. The Acro:-Tnc:e Crou:lu rcliOpmont (ACE)	 ur.cd to service the v.ater— system lr.:
dJ 511,fcctc.d mid hioassriyed at rcf;ul.;tr interval:..
steri lized traiv-S(ir .end t( . ,-.t equiJ,mrmnt t:;;r imud wherever
1;acte)- i'll popol;lt:ioi1:; in	 the	 r;:nyc of 10 to lU{) colmic; licr r.11	 condlme l	 to	 l;c±
found in tilt:	 : y:;tc: mu 	 at ; pp. rent-l) , run-10',11 intcl'v,^ ls•
Laltor;ll,m y testing of the: contamin 1ti.nL ol • i;nlli ,ul r.ho\x!d th;it i.t was c,•1J^::hlu
of t;ro,;t.h o'1 a n:,t:r3cnt su]lllly th,'A tars Ilnde tectabl,. by otir standard hbor;it,,ry
analyti e.Il prozedures. Inoclll:, of the	 were i.t ► cuazted ili f la:;l:r:
u.1t':.i.tlin	 1)i,;b pert; :v delnni •r.ed t-.,.—Uo r ) (tho	 W;1t(-r u;.ud to prlm--^ ; ► nil fof--t
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c:ont-anii.natin,; orranir;l:l ►ins ir!j,licated in all cn::cs.
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The ructirri ng bacterial contruni nant found In tihe tSlor^ t c i i t ,.	 *, `,
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control witl ► e1lt the c!rlllloyllicllt of strict sterile procedure.:; or chcimical
througho:lt. tl.(t r;pac • cc•raft in.muf;;cturc and te;r cycle.
	 Addi.t:ionnl tre;itnlent o:
thct water ,'y. Lcm by 1 ►:hlooc'n:lt.ion or other Inealln would bc. rc • clilirecl to control t:hc•
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Identification of a recurring bacterial contaminant in a spacecraft watering system

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