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木糖异构酶基因xylA表达载体构建
Authors
叶菁
杨柳
+3 more
袁振宏
许敬亮
陈小燕
Publication date
1 January 2014
Publisher
Abstract
以大肠埃希菌 MG1655的基因组为模板,通过PCR扩增获得木糖异构酶基因xylA。利用敲除编码对基因转录起负调控作用的lacIq基因的大肠埃希菌/谷氨酸棒杆菌穿梭质粒pEC-XK99E,酶连后转化大肠埃希菌BL21和谷氨酸棒杆菌ATCC 13032。成功构建出了具有大肠埃希菌BL21表达活性的木糖异构酶表达载体Ptrc-xylA
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Institutional Repository of GuangZhou Institute of Energy Conversion, CAS
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oai:ir.giec.ac.cn:344007/21567
Last time updated on 17/12/2019
Institutional Repository of GuangZhou Institute of Energy Conversion, CAS
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:ir.giec.ac.cn:344007/21569
Last time updated on 17/12/2019
Institutional Repository of GuangZhou Institute of Energy Conversion, CAS
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:ir.giec.ac.cn:344007/31366
Last time updated on 04/12/2021