Institute for Medical Research and Occupational Health
Abstract
The results of double diffusion analysis of the native and dialyzed Ammodytes viper venom are presented. It is found that a group of antigens was lost from the native venom during dialysis procedure, another group being insoluble in distilled water was eliminated from the aqueous venom solution by filtration. The approximative number and characteristics of precipitation lines produced by the native venom or isolated venom fractions with the immune horse serum in agar-gel are reported.Izvedena je imunoprecipitaciona difusiona analiza toksina poskoka (Vipera ammodytes a.) u agar gelu (Sl. 1). Antigeni upotrebljeni u eksperimentima bili su: (1) sirovi otrov poskoka, antigeni otrova koji ne difundiraju kroz celofansku membranu (2), u vodi topivi dio (3), te u dejoniziranoj vodi netopivi antigeni iz nativnog otrova (4). Kao antivenin (S23) upotrebljen je koncentrirani imuni konjski serum. Iz rezultata difuzione analize vidi se, da se jedna grupa antigena (d) sirovog otrova gubi iz vodene otopine u toku dijalize, dok se iz dijaliziranog otrova mogu filtracijom odstraniti u vodi netopivi antigeni (i). Određen je približni broj precipitacionih linija odnosno antigenih komponenata u nativnom otrovu i iz njega izoliranim frakcijama
