: Vulva hygiene is clean the vulva and the surrounding area in woman who werepatients at parturition or can not do it alone. Vulva hygiene also aims to prevent infection, tostitch the perineal wound healing and for the cleanliness of the perineal. The purpose of thisstudy analyzed the relationship vulva hygiene with prevention of perineal wound infection inthe mother post partum in the Hospital Pancaran Kasih GMIM Manado. The researchdesign used in this research is descriptive analytic design with cross sectional approach. Theresults using the Chi-Square test values obtained significant vulva hygiene, namely P = 0,001< á = 0,05. The conclusions of this research is vulva hygiene relationship with theprevention of wound infection of the perineal in the mother post partum. Suggestion for otherresearchers are expected to conduct further research in orderto be aware of factors otherfactors that affect the prevention of perineal wound infection in the mother post partum