Kajian Reversibilitas Interaksi Marmin Terhadap Reseptor Histamin H1, Asetilkolin Muskarinik Ach-m3 Dan β2-adrenergik

Abstract

Previous studies concluded that amarmin(7 - (6 \u27, 7\u27-dihidroxygeranyl-oxy) coumarin) shown to inhibit isolated Guinea-pig tracheal smooth muscle contraction through its effect as an H1and Ach-M3receptor\u27s antagonist. Marmin also slightly increases tracheal smooth muscle relaxation induced by isoproterenol through its interaction on β2-adrenergic receptor. The purpose of this experimental study was to evaluate the reversibility of marmin interaction on H1, Ach-M3 and β2-adrenergic receptors.The study conducted using in vitro isolated-trachea experimental. Reversibility studies performed after marmin (10 and 100) μM treatment on tracheal smooth muscle contraction/relaxation induced by agonists (histamine, methacholine and isoproterenol). Trachea was washed for 30minuteswith Krebs buffer solution replacement every five minutes. Furthermore, trachea contracted or relaxed with series histamine (10-8 – 10-3) M; methacholine (10-8 – 10-3) M and isoproterenol (10-7 – 3.10-3) M. Data were expressed as mean±SEM. The pD2values are derived from the negative logarithm to base 10 of the agonist\u27s concentration which cause a half maximal response to the contraction or relaxation.The results showed that the marmin interaction on H1, Ach-M3, and β2-adrenergic receptors are reversible. One-way Anova concluded that histamine, metacholine and isoproterenol pD2 value before and after marmin (10 and 100) μM treatments are not different statistically (p>0.05)