: Diarrhea is one of the main cause of infant mortality in developing countries. Blastocystis spp can cause acute infection as well as manifest as chronic diarrhea in infants due to damaged intestinal mucosa. This study aimed to detect Blastocystis spp in the feces of children with diarrhea. This was a descriptive study with a cross-sectional design. Population consisted of all feces of children suffering from diarrhea. There were 33 samples in this study examined with the copro Elisa test. The results showed that of the 33 samples there were 60.6% of negative criteria and 39.4% of positive criteria. The highest percentage age group with diarrhea was ≤1 year of age (48.5%), while the lowest one was age group of 5-9 years (9.1%). Male sex was the dominant one. Conclusion: Most of the faeces of children with diarrhea belonged to the negative criteria tested with Copro Elisa