Effectiveness of Aloe Vera Gel and Coconut Water as a Bioregulator on Seed Germination of Dendrobium Orchid

Abstract

The study aimed to determine the effect of concentration of coconut water and aloe vera gel on seed germination of Dendrobium orchid was done in Tissue Culture Laboratory Unmas Denpasar, from the month of April to August 2010. The research method using nested completely randomized design with 3 replications. Treatment additions bioregulator on VW medium consisting of coconut water with a concentration of 0 ml/l, 150 ml/l, 200 ml/ l, 250 ml/l and 300 ml/l, and aloe vera gel with a concentration of 0 g/l, 5 g/l, 10 g/l, 15 g/l and 20g/l.The results showed that there were no initial differences in germination and percentage germination between VW medium given coconut water and given aloe vera gel. VW medium fed coconut water 150 ml/l and 200 ml/l gave the early germination and highest germination percentage. While the VW medium given aloe vera gel 5 g/l and 10 g/l gives the fastest initial germination and the highest germination percentage.To speed up germination and increase the percentage of seed germination of Dendrobium in the culture medium in-vitro with VW, may be the addition of coconut water 150 -200 ml/l or with leaves of aloe vera gel with a concentration of 5-10 g /