: Nosocomial infections is an infection acquired in the hospital in patients treated for at least 72 hours (3x24 hours). Environmental infection caused by bacteria from an existing object within the hospital. Microorganisms are often potentially causing nosocomial infections are Proteus sp., Escherichia colii, Staphylococcus aureus, Candida albicans, and Pseudomonas aeruginosa. Objective: to know the most aerobic bacteria and the pattern of aerobic bacteria in Pediatric Intensive Care Unit at BLU Dr Prof. Dr R. D. Kandou. Methods: 24 swab samples were taken on the surface of the tool, treatment rooms and 6 samples of air space. Identification of bacterial cultures taken on an agar medium, gram staining and biochemical tests. Results: identified bacteria obtained 11 types of microorganisms, such as Bacillus subtilis, Staphylococcus sp., Enterobacter agglomerans, Serratia rubidaea, Enterobacter aerogenes, Enterobacter cloacae, Coccus Gram-negative, Klebsiella pneumoniae, Candida sp., Pseudomonas sp., Streptococcus sp. Conclusion: Gram negative bacteria are bacteria that are found in the Pediatric Intensive Care Unit. Staphylococcus sp., and Pseudomonas sp., a common bacteria that cause nosocomial infections with Staphylococcus sp., a bacterium that is most found