Papaya (Carica papaya L.) is one of the most important fruit plant in the fulfillment ofcalcium and source of vitamins A and C. This research aimed to examine diuretics effecct inmale rats and identification of chemical contents in the ethanol extract of papaya seed. Asmuch 15 test animals were divided into 5 groups, that is negative control (0,5% CMCsuspension), positive control (suspension furosemide 5,04 mg / kgBB), dose of extract 0,03g/kgBB dose of extract 0,06 g/kgBB, and dose of extract 0,12 g/KgBB. The test is done bymeasuring of diuretics effect the urine volume incurred for 6 hours. Data obtained wereanalyzed with SPSS ver.20, one way ANOVA test and advanced test that is Duncan test.Statistical analysis showed a significant difference (α <0.05) between the negative controland positive control and dose of extract. Phytochemical screening results obtained arealcaloids and saponins, but is efficacious as of diuretics is an alcaloid. Based on these resultswe can conclude the ethanol extract of papaya seeds have a diuretic effect in male white ratswistar strain and identified the active compounds contained alcaloids and saponins
