背景和目的：PKM2作为丙酮酸激酶（pyruvatekinaseisoenzyme，PK）家族成员，在肿瘤中呈现高表达，并且在肿瘤能量代谢过程中起到关键作用。近期研究发现，PKM2还具备转录因子活性，参与调控细胞增殖与迁移侵袭，在恶性肿瘤的发生发展中发挥重要作用。我们研究发现，胃癌细胞株BGC823及AGS中基因沉默PKM2后，促进了顺铂诱导的细胞凋亡，由此表明PKM2可以减低胃癌细胞对化疗药物的敏感性，但具体机制有待探索，我们的研究旨在明确基因沉默PKM2在胃癌细胞凋亡及耐药方面的作用及相关分子信号通路。 方法：首先，运用短发夹RNA（shRNA）稳定转染胃癌细胞株BGC823和AGS，抑...Background and Aims: The M2 splice isoform of pyruvate kinase (PKM2) as a member of pyruvate kinase isoenzyme family , highly expressed in tumor cells, and plays an important role in the process of tumor metabolism. The latest study finds that PKM2 has an activity of transcription factor , participate in regulation of cell proliferation and migration, and plays an important role in the emergence ...学位：医学硕士院系专业：医学院_内科学学号：2452012115313
