目的:初步研究膀胱间充质干细胞促进膀胱癌生长及迁移的机制。方法:1.体外分离、培养并鉴定膀胱间充质干细胞;2.在体外实验中,以WST-1测定膀胱间充质干细胞培养上清液对于膀胱癌细胞生长的影响,并以划痕实验测定膀胱间充质干细胞培养上清液对于膀胱癌细胞迁移能力的影响;3.在动物模型中测定膀胱间充质干细胞对于膀胱癌细胞生长和迁移能力的影响;4.以WESTERN-BLOT检测相关信号通路关键蛋白表达的变化以研究膀胱间充质干细胞促进膀胱癌生长及迁移的机制:结果:1.膀胱间充质干细胞表达CD105,CD73和CD90,基本不表达CD45,CD34,CD14,CD79和HLA-DR,具有三系分化能力,表达表...Objective:Preliminary study on mechanisms of urinary bladder mesenchymal stem cells promote cell growth and migration of urothelial carcinoma cells line 5637. Methods:1.Isolation,cultivation and identification of urinary bladder mesenchymal stem cells from bladder tissue. 2.Determinate urinary bladder mesenchymal stem cell culture supernatant impacts urothelial carcinoma cells line 5637 on growth ...学位:医学硕士院系专业:医学院_外科学学号:2452012115318