昆虫细胞杆状病毒表达系统(BEVS)是真核表达系统的一种，以一种无需额外辅助的重组昆虫杆状病毒作为表达载体，目前已被应用来表达不同来源的基因，如细菌、病毒、植物和哺乳动物的基因。它具有表达产量高，蛋白经过完善的后处理过程，如内含子剪切、糖基化等特点。其原理是通过外源基因置换病毒的核多角体蛋白基因，使外源基因在核多角体蛋白启动子下高效表达。本论文中采用BEVS表达HIV-1包膜蛋白，目的在于弥补原核表达系统不能表达大片段的、高度糖基化的重组蛋白；并探索其应用在检测试剂中的前景。Baculovirus / insect cell expression system (BEVS) is a kind of Eukaryotic expression system. This is a helper-independent recommbinant virus vector that has used to express genes from many sources: bacteria, viruses, plant and mammals. It has high level expression and perfect post-transcription modification, such as intron cutting, glycosylation. The principle of BEVS is replacing the polyhedrin ...学位：暂缺院系专业：暂缺_暂缺学号：que00007
