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The Study of Ultrastructure、ATP、Calcium and RNA-Seq in fertile and sterile anthers of Lycium Barbarum L.

Abstract

高等植物的雄性不育是杂种优势利用的基础,避免了去雄的繁杂程序。植物雄性不育的种类很多,形成原因也很复杂,对雄性不育机理的探索一直是一个热门研究领域。枸杞雄性不育系是近年来新发现的一种不育类型,具有很大应用潜力,为了明确其雄性不育发生的机制并更好地开发利用这一宝贵种质资源,我们从结构、生理和分子生物学等几个方面展开了研究。 本研究以可育系宁杞1号和不育系宁杞5号为实验材料,用超微结构、ATPase和Ca2+的细胞化学定位,以及转录组和数字基因表达谱研究了枸杞花药发育的特征并比较了不育花药形态、结构和基因表达的差异。主要研究结果如下: (1)超微结构研究结果显示,不育花药最早出现的结构异常是在...The male sterility (MS) of higher plants is the basis for utilization of heterosis. However, there are many types of MS and the reasons caused to it are very complex. The mechanism of male sterility is still unclear, and the research of MS has been a hot research field for a long time. Ningqi 5 is a new type of MS found in Lycium Barbarum L. in 2005. In order to figure out the mechanism of MS a...学位:理学博士院系专业:生命科学学院_发育生物学学号:2162012015381

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