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Establishment of An Effective System to Develop Avian Polyclonal Antibodies and Its Primary Application

Abstract

小鼠单克隆抗体技术已成为生物医药领域不可或缺的研究工具,在疾病的诊断和治疗领域也得到广泛应用。然而,由于小鼠自身免疫系统的局限,某些抗原或表位,特别是同为哺乳动物的人体抗原在小鼠体内可能因为种属相近而不易产生好的免疫应答,给制备相应的小鼠单克隆抗体造成一定的困难。因此,有必要基于其它种属动物开发新的抗体制备技术。禽源抗体技术也称卵黄抗体技术(简称IgY技术),是用免疫原刺激禽类动物,产生相应的特异性抗体,该抗体会转运、储存在卵黄中,经特殊的提取技术,可从卵黄中获得大量特异性的多克隆抗体的一种抗体制备技术。相较于哺乳动物的IgG抗体,禽源IgY抗体的优点有:1)减少对动物的伤害,符合日渐严格的动...Mouse monoclonal antibodies have become an indispensable biomedical research tool since appeared. And it has been wildly used in field of diagnosis and treatment of diseases. However, due to the limitation of mouse’s immune system, it’s hard for some antigen or epitope in particular with some human antigens to make mouse produce a good immune response, and it may cause some trouble in preparing mo...学位:理学硕士院系专业:生命科学学院_生物化学与分子生物学学号:2162013115258

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