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Molecular cloning, expression and immunohistochemical localization of Ac-ALMP, an astacin-like metalloprotease from Angiostrongylus cantonensis

Abstract

广州管圆线虫病是一种人畜共患的寄生虫病。它是因人进食含有广州管圆线虫感染期幼虫的食物或水源而引起的。随着人们饮食习惯的改变和水陆交通的日趋发达,该病的流行呈逐年上升的趋势,目前尚无理想的特效药对该病进行防治。虾红素金属蛋白酶是金属蛋白酶家族的一员,研究发现,其在寄生虫寄生生活中发挥一系列不同的生物学功能。但是目前国际上,关于广州管圆线虫虾红素金属蛋白酶的报道几乎没有。 本研究为探索广州管圆线虫虾红素金属蛋白酶在虫体中的功能,对其基因进行调取,我们通过虾红素金属蛋白酶EST序列设计引物进行RACE克隆,最终获得广州管圆线虫虾红素金属蛋白酶全长基因,并将其命名为Ac-ALMP(Astacin-l...Angiostrongyliasis cantonensis is one of zoonotic parasitic disease. Human or animals can infect because consuming raw or undercooked intermediated hosts, contaminated vegetables. In recent years, the incidence of angiostrongyliasis has become increasingly significant with the change of diet habits along with development of transport facilities in water and land. However, there are not effective m...学位:理学硕士院系专业:生命科学学院_生物化学与分子生物学学号:2162013115459

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