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Detection and Analysis of Antibiotic Resistance Genes in a Wastewater Treatment Process

Abstract

目前,随着在越来越多的污水样品和处理后的生物残渣中检测到耐药菌和抗性基因,人们已逐渐认识到污水处理厂是这二者的一个主要容留场所。环境中的这些抗性基因可以作为感染源水平转移到人类机体微生物中,也因此导致了抗生素耐药性的扩大化。由于胞外基因分子可以在水环境中保持相当长时间的生物活性,而目前关于污水中胞外基因的报道甚少,本文将着重调查典型的污水处理工艺——膜生物反应器中的胞外基因,并在此基础上进一步考察膜生物反应器出水中的胞外抗性基因(尤其是磺胺甲恶唑抗性基因)可能对细菌产生的抗性基因转化。 首先,本文改进和验证了一项同时提取胞内和胞外基因的方法,结果显示胞外基因的回收率为43-67%且几乎没有细...Nowadays, wastewater treatment plants have been gradually recognized as a reservoir for antibiotic resistant bacteria and antibiotic resistance genes(ARGs), which were found in lots of wastewater samples and treated biosolids. These environmental ARGs then could serve as a source and be horizontally transferred to human-associated bacteria and thus contribute to antibiotic resistance proliferation...学位:工学硕士院系专业:化学化工学院_化学工程学号:2042012115417

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