拟穴青蟹(Scyllaparamamosain)是我国东南沿海重要的经济蟹类。本文以拟穴青蟹为研究对象,采用动物生理学、分子生物学、生物信息学等方法和手段,研究了3种小分子神经肽即红色素聚集激素(Redpigmentconcentratinghormone,RPCH)、亮氨酸-脑啡肽(Leucineenkephalin,Leu-ENK)和甲硫氨酸-脑啡肽(Methionineenkephalin,Met-ENK)的结构、特征和生殖调控功能。主要结果如下: (1)构建了卵巢成熟期青蟹胸神经团差异表达基因的cDNA消减文库。该消减文库包含了410条表达序列标签(Expressedsequence...Mud crab, Scylla paramamosain (Estampador, 1949) is one of the important marine economical animals in southern China. All the animal physiology, molecular biology and bioinformatics research methods were used to study the structure, characteristics and reproductive regulation function of red pigment concentrating hormone (RPCH), leucine enkephalin (Leu-ENK) and methionine enkephalin (Met-ENK) in m...学位:理学博士院系专业:海洋与地球学院_海洋生物学学号:2242009015326