Bertrand, Carole

Cossu-Leguille, Carole

Giambérini, Laure

Koehle-divo, Vanessa

Pain-Devin, Sandrine

Simonin, Cécile

Sohm, Bénédicte

International audienceAbstract: The rapid development of nanotechnologies and the increased use of nanomaterials in everyday life products have raised the question of the potential release of nanoparticles into the aquatic environment. Their fate and effect in natural ecosystems are not currently well understood but harmful effects of nanoparticles have been demonstrated at low concentrations in some freshwater and marine species. Cerium dioxide nanoparticles (nCeO2) are produced in large quantities and used in products on many different fields, such as automotive or optic. Because of their widespread use in daily products, they are listed in OECD priority list of manufactured nanomaterials for testing. Some studies were conducted to assay various enzymatic biomarkers and show the potential of nCeO2 to impact oxidative defenses and cellular membrane stability but only few studies were performed on their genotoxic potential, that’s why we performed an approach coupling genotoxic and enzymatic biomarkers. The aim of the present work was to evaluate the genotoxic and physiological effects of nCeO2 on a widespread freshwater bivalve Corbicula fluminea by using comet assay and a multi-enzymatic biomarker approach. Exposure to low concentrations of nCeO2 (10 and 100 µg.L-1) during a short term experiment (2 and 6 days) was set up. DNA damages appeared significantly more pronounced compared to control for both concentrations tested as earlier as two days of exposure and seems to increase with time. Some enzymatic biomarkers of oxidative defenses tend to increase after 6 days of exposure but only caspase pathway may be correlated with DNA observed damages. In this study, time and concentration effects of nCeO2 were highlighted by coupling genotoxic and cellular biomarker assessments

Koehle-Divo, Vanessa

Sohm, B.

Giamberini, L.

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