Aikeshev, B.

Brooks, P.

Ishchenko, A.

Mazouzi, A.

Morera, S.

Saparbaev, M.

Vigouroux, A.

Proceedings of the National Academy of Sciences

PubMed

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Insight into mechanisms of 3'-5' exonuclease activity and removal of bulky 8,5'-cyclopurine adducts by apurinic/apyrimidinic endonucleases

Significance
          
            Oxidative DNA damage has been postulated to play an important role in human neurodegenerative disorders and cancer. 8,5′-
            cyclo
            -2′-deoxyadenosine (cdA) is generated in DNA by hydroxyl radical attack and strongly blocks DNA replication and transcription. Here we demonstrate that cdA adducts at 3′ termini of DNA can be removed by 3′-5′ exonuclease activity of the apurinic/apyrimidinic (AP) endonucleases:
            Escherichia coli
            Xth and human APE1. The crystal structure of bacterial AP endonuclease in complex with DNA duplex provides insight into the mechanism of this activity. This new repair function provides an alternative pathway to counteract genotoxic effect of helix-distorting DNA lesions.
          </jats:p

Abdelghani Mazouzi

Armelle Vigouroux

Bulat Aikeshev

Philip J. Brooks

Murat K. Saparbaev

Solange Morera

Alexander A. Ishchenko

Crossref

Insight into mechanisms of 3′-5′ exonuclease activity and removal of bulky 8,5′-cyclopurine adducts by apurinic/apyrimidinic endonucleases

ISHCHENKO, A.

Archive Ouverte en Sciences de l'Information et de la Communication

http://dx.doi.org/10.1073/pnas.1305281110

Insight into mechanisms of 3'-5' exonuclease activity and removal of bulky 8,5'-cyclopurine adducts by apurinic/apyrimidinic endonucleases

Abstract

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Crossref

Archive Ouverte en Sciences de l'Information et de la Communication