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research
Dichloroacetate affects proliferation but not apoptosis in canine mammary cell lines
Authors
Hugo Murua Escobar
Susanne C. Hammer
+5 more
Tatjana P. Harting
Anaclet Ngezahayo
Ingo Nolte
Patrik Schadzek
Mandy Stubbendorff
Publication date
1 January 2017
Publisher
San Francisco : Public Library of Science
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Abstract
Targeting mitochondrial energy metabolism is a novel approach in cancer research and can be traced back to the description of the Warburg effect. Dichloroacetate, a controversially discussed subject of many studies in cancer research, is a pyruvate dehydrogenase kinase inhibitor. Dichloroacetate causes metabolic changes in cancerous glycolysis towards oxidative phosphorylation via indirect activation of pyruvate dehydrogenase in mitochondria. Canine mammary cancer is frequently diagnosed but after therapy prognosis still remains poor. In this study, canine mammary carcinoma, adenoma and non-neoplastic mammary gland cell lines were treated using 10 mM Dichloroacetate. The effect on cell number, lactate release and PDH expression and cell respiration was investigated. Further, the effect on apoptosis and several apoptotic proteins, proliferation, and microRNA expression was evaluated. Dichloroacetate was found to reduce cell proliferation without inducing apoptosis in all examined cell lines. © 2017 Harting et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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Last time updated on 12/02/2018
Institutionelles Repositorium der Leibniz Universität Hannover
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Last time updated on 05/08/2017